 |
/ Home
/ Web Directory / Science / Biology / Biochemistry and Molecular Biology / Methods and Techniques / PCR (134)
Sub-Categories in PCR
Web Sites in Category PCR
Displaying: 1 – 50 (82 total) Next Page >>
3' RACE PCR - This technique is used to obtain the 3'end of a cDNA. Protocol by R Hastings, Biochemistry, University of Nottingham Medical School, UK.
A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document) - Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
Adjuvants in PCR Reactions - Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.
Alu-PCR Hybridization - A report and protocol, from Fondation Jean Dausset.
Anchor Probes for Comparative Mapping of Grass Species - Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.
Attotron Biosensor Corporation - R & D company for development of biosensors and related products for the research and educational markets.
BioGuide - PCR - Basics of hot start, touchdown, AP and RAPD, primer selection, design programs and references, from the Weizman Institute of Science, Rehovot, Israel.
Biosource molecular methods booklet - 56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).
C. elegans Gene Knockout Project - Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.
Cleaved Amplified Polymorphic Sequence - Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.
Degenerate PCR - The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
Degenerate PCR - An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.
Design Oligonunucleotide Primers - Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.
Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy - In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences - RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.
Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file) - P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.
DNALC: PCR Animation - An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.
Effect of PCR Buffer on multiplex PCR (PDF) - Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)
Fidelity of DNA Polymerases for PCR - Article by PN Hengen from TIBS 1995
FISH Guide and Troubleshooting - Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.
General Notes on Primer Design in PCR - Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published on the domain of Brinkmann Instruments.
GenHunter - Manufacturer of material for differential display PCR in Nashville, Tenn USA.
HiFi DNA - HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
Hot Start PCR - Description of the technique and the reasons to use it, from the web site of Bio Can, a company manufacturing materials for PCR.
Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay - P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc.
Innovative Tools for Amplification - Notes about PCR applications, principles, research results, from Roche.
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation - Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
Inverse PCR for PAC-end sequencing - To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.
Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis - Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
Inverse PCR protocol - step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
Kary B. Mullis - Autobiography - The originator of PCR, from the Nobel e-museum web site.
Kary Mullis - Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
Life at High Temperatures - Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained.
Long PCR Protocol - Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
MJ Research - Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.
Molecular Staging, Inc. - Company promoting diagnostic tests for genetic analysis; rolling circle amplification technology (RCAT), developed for amplifying signals from DNA, RNA and proteins, from Westport, CT.
Multiplex PCR - A new PCR strategy that enables the amplification of multiple DNA targets in one run. From Boston University.
Mutation Detection Systems - SSCP Analysis - Discussion of the technique and variations, literature references. By S McQuaid, Institute of Child Health, University College London, UK.
Nematode ITS1 Size Variation - Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes.
Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler - Basic discussion of the optimization of PCR, from the web site of Brinkmann Instruments.
Optimizing Multiplex and LA-PCR with Betaine - LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.
Optimizing PCR Protocols - Brief guidelines. From the Jackson Laboratory, University of Maine, USA.
PCR Amplification of cDNA Segments by 2 Stage Nested PCR - Protocol from the method database of NIH, USA.
PCR and multiplex PCR guide - Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.
PCR animated - Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
PCR Animation - Explaining how Polymerase Chain Reaction (PCR) works. From Dolan DNA Learning Center, Cold Spring Harbor Laboratory, USA.
PCR method protocols - Protocols for PCR posted by the Helen Donis-Keller Laboratory.
PCR primer design and reaction optimisation - Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.
PCR Project - Presentations from the University of California at Berkley on PCR, both current research reports and reviews.
PCR Protocol - Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
Displaying: 1 – 50 (82 total) Next Page >>
Related Categories:
Science > Biology > Biochemistry and Molecular Biology > Gene Expression > Products and Services
Editors:
lfischer
|
|
|
|
